Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: Baseline data in patients with osteosarcoma.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: Univariate and multivariate analysis of patient characteristics.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: Prognostic value of PROSER2 in patients with osteosarcoma. (A) Survival probability shown by Kaplan-Meier plotter in patients with osteosarcoma. Subgroup analysis of overall survival in osteosarcoma for (B) non-metastasis, (C) males, (D) distal tumor region and (E) no primary site progression. PROSPER2, proline- and serine-rich 2; HR, hazard ratio.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: PROSER2 knockdown inhibits viability of osteosarcoma cells. (A) Expression of PROSER2 was determined by western blot analysis in four pairs of clinical specimens. ** P<0.01. (B) PROSER2 levels were determined by western blot analysis in hFOB1.19, MG63 and U2OS cells. *** P<0.001 vs. hFOB1.19. (C) MG63 and U2OS cells were infected with PROSER2 and control shRNA lentivirus for 24 h and PROSER2 expression was determined by western blotting analysis. * P<0.05, *** P<0.001. (D) Viability of MG63 and U2OS cells infected with PROSER2 or Ctrl shRNA lentivirus and cultured for 24, 48 and 72 h was assessed by MTT assay. ** P<0.01, *** P<0.001 vs. Ctrl shRNA. PROSPER2, proline- and serine-rich 2; Ctrl, control; T, tumor tissue; C, paratumor tissue; OD, optical density; sh, short hairpin.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Knockdown, Expressing, Western Blot, Infection, Control, shRNA, Cell Culture, MTT Assay

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: PROSER2 knockdown inhibits proliferation and migration of MG63 cells. MG63 cells were infected with PROSER2 and Ctrl shRNA lentiviruses for 24 and 48 h. (A) Wound healing assay. Magnification, x40. (B) Migration and (C) invasion assay were performed to investigate the role of PROSER2 on proliferation and migratory ability of MG63 cells. Magnification, x100. (D) Wound closure, migratory and invasive cells. * P<0.05 and ** P<0.01 vs. Ctrl shRNA. PROSPER2, proline- and serine-rich 2; Ctrl, control; sh, short hairpin.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Knockdown, Migration, Infection, shRNA, Wound Healing Assay, Invasion Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: PROSER2 is involved in the Wnt/Ca 2+ signaling pathway in MG63 cells. (A) MG63 cells were infected with PROSER2 and Ctrl shRNA lentivirus for 24 h. The expression of Wnt5a, COX2, MMP-2 and MMP-9 was detected by western blot analysis in PROSER2 and Ctrl shRNA lentivirus-infected MG63 cells. (B) Histograms of relative expression of Wnt5a, COX2, MMP-2 and MMP-9. (C) Nuclear expression of NFATc1 and cytoplasmic CNA were detected by western blotting analysis in PROSER2 and Ctrl shRNA lentivirus-infected MG63 cells. (D) Histograms of relative expression of NFATc1 and CNA. ** P<0.01. (E) Confocal microscopy imaging. Intracellular Ca 2+ was measured by incubation with the Ca 2+ selective fluorescent indicator, Fura-2 AM. Scale bar, 10 µM. PROSPER2, proline- and serine-rich 2; NFATc1, nuclear factor of activated T-cells 1; CNA, catalytic subunit of calcineurin; Ctrl, control; sh, short hairpin; COX, cyclooxygenase; MMP, matrix metalloproteinase.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Infection, shRNA, Expressing, Western Blot, Confocal Microscopy, Imaging, Incubation, Control

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: Interference with PROSER2 promotes VIVIT-induced inhibition of NFATc1 in MG63 cells via suppressing nuclear localization of NFATc1. (A) MG63 cells were transfected with PROSER2 or Ctrl shRNA lentivirus and treated with 10 µM VIVIT for 24 h. The nuclear expression of NFATc1 and lamin B1 was detected by western blotting. Lamin B1 was used as a nuclear internal reference protein. The expression of Wnt5a, COX2, MMP-2 and MMP-9 were determined by western blotting analysis. (B) Histograms of relative expression of NFATc1, Wnt5a, COX2, MMP-2 and MMP-9. ** P<0.01 and *** P<0.001. PROSPER2, proline- and serine-rich 2; NFATc1, nuclear factor of activated T-cells 1; Ctrl, control; sh, short hairpin; COX, cyclooxygenase; MMP, matrix metalloproteinase.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Inhibition, Transfection, shRNA, Expressing, Western Blot, Control

Journal: Experimental and Therapeutic Medicine

Article Title: PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells

doi: 10.3892/etm.2022.11686

Figure Lengend Snippet: Interference with PROSER2 promotes apoptosis of MG63 cells via suppressing nuclear localization of NFATc1 mediated by CsA. (A) MG63 cells were infected with PROSER2 and Ctrl shRNA lentivirus and treated with 3 µg/ml CsA for 24 h. Expression of NFATc1 was detected by western blot analysis. Lamin B1 was used as a nuclear internal reference protein. *** P<0.001. (B) Apoptosis was determined by fluorescence-activated cell sorting assay in PROSER2 and Ctrl shRNA lentivirus-infected MG63 cells with or without CsA. * P<0.05, ** P<0.01 and *** P<0.0001. PROSPER2, proline- and serine-rich 2; NFATc1, nuclear factor of activated T-cells 1; CsA, cyclosporine A; sh, short hairpin; Ctrl, control; PI, Propidium iodide; FITC-A, fluorescein isothiocyanate; Q, quadrant.

Article Snippet: The transduction of PROSER2 and control shRNA lentiviral particles (generation system used was 3rd) was performed according to the manufacturer's protocol (cat. no. TL306312V, OriGene Technologies).

Techniques: Infection, shRNA, Expressing, Western Blot, Fluorescence, FACS, Control